Research Article

Mesenchymal Stromal Cells from Osteoarthritic Synovium Are a Distinct Population Compared to Their Bone-Marrow Counterparts regarding Surface Marker Distribution and Immunomodulation of Allogeneic CD4+ T-Cell Cultures

Figure 4

Evaluation of MSC/CD4+ T-cell interaction upon T-cell stimulation. (a) Representative photographs at d2, d4, and d5 of coculture for bone-marrow (BM) and synovial membrane (SM) derived MSCs are displayed. Index: 100 μm. (b) Representative histograms from one CFSE assay (total triplicate assays ) for unstimulated CD4+ T-cells, unstimulated CD4+BM-MSC and SM-MSC cocultures, and the respective CD3/CD28 stimulated cultures. (c) The histogram displays the mean percentage of proliferated cells in five triplicate CFSE assays (see Methods for gating). and .
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