Research Article

FTIR Spectroscopic and Molecular Analysis during Differentiation of Pluripotent Stem Cells to Pancreatic Cells

Figure 2

Representative images of immunofluorescence on mouse pluripotent stem cells (mPSCs) and Differentiated Pancreatic Cells (DPCs). The pluripotency markers Nanog and SOX2 were immunodetected on mPSCs cultures, evidencing the pluripotency state. The immunoexpression of pancreas proteins (Insulin and Glucagon) in the Embryoid Bodies (EBs) corroborated the obtention of pancreatic cells ( with 3 biological replicates, 100x, Insulin 200x).