TBX3 Knockdown Decreases Reprogramming Efficiency of Human Cells

Figure 1

Reprogramming fibroblasts and keratinocytes with a TBX3 knockdown. (a) Infection of fibroblasts and keratinocytes with a TRIPZ Human TBX3 shRNA lentivirus, inducible via doxycycline addition. (b) Reprogramming time scheme with fibroblasts (left) and keratinocytes (right) along with starting cell numbers and experiment time points. (c) TBX3 knockdown during reprogramming of fibroblasts at day 0 (seeding infected cells on feeder layer), day 2, day 4, day 17, and day 25 (last day on feeder cells before transfer to feeder-free system on Matrigel). Control is not treated with doxycycline. (d) TBX3 knockdown during reprogramming of keratinocytes at day 0 (seeding infected cells on feeder layer), day 2, day 5, and day 22 (last day on feeder cells before transfer to feeder-free system on Matrigel). Control is not treated with doxycycline.

(a)

(b)

(c)

(d)