Extracellular Matrix-Dependent Generation of Integration- and Xeno-Free iPS Cells Using a Modified mRNA Transfection Method

Figure 4

Genome-wide gene expression profiling of the mRNA-iPSCs. (a-b) Scatterplot analysis showed that the global gene expression pattern of mRNA-iPSCs is similar to that of H9-hESCs (a). However, the gene expression patterns are clearly different between mRNA-iPSCs and their parental cells, fibroblasts (b). (c) Heatmap analysis indicated that the expression patterns of 100 hESC-enriched genes and 100 human fibroblast-enriched genes (Supplementary Table  4) in mRNA-iPSCs were similar to those in H9-hESCs but not fibroblasts. (d-e) PluriTest analysis showed that mRNA-iPSCs and other previously established iPS cells (UNFiPSC1 and ANiPSC1) [18] were clustered with H9-hESCs in the pluripotent group, whereas fibroblasts and other primary cells were clustered in the nonpluripotent group. (f) Gene profiling-based hierarchical clustering analysis demonstrated the close association of the mRNA-iPSCs with H9-hESCs but not with the primary cells (fibroblasts).

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