Extracellular Matrix-Dependent Generation of Integration- and Xeno-Free iPS Cells Using a Modified mRNA Transfection Method

Figure 5

Analyses of the methylation in the Oct4 and Nanog promoters and of chromosomal abnormality in the mRNA-iPSCs. (a) The bisulfite sequencing data indicated that the promoters of the Oct4 and Nanog genes in the mRNA-iPSCs were largely demethylated, similar to the methylation status of these promoters in hESCs. In contrast, their original cells, fibroblasts, were hypermethylated at these promoters. (b) G-banding analysis showed that no apparent chromosomal abnormality was generated during reprogramming and extended culture (for 35 passages) of the mRNA-iPSCs in our ECM-based xeno-free/feeder-free hPSC culture system.

(a)

(b)