Extracellular Matrix-Dependent Generation of Integration- and Xeno-Free iPS Cells Using a Modified mRNA Transfection Method

Figure 6

Analyses of pluripotency in vitro and in vivo. The mRNA-iPSCs were subjected to spontaneous differentiation via EB culturing, and the expression levels of representative markers of the ectoderm (Nestin and Class III β-tubulin (Tuj1)), mesoderm (SMA and PECAM), and endoderm (AFP and FoxA2) lineages were examined. Scale bar: 100 µm. (b) Teratomas including structures and derivatives of the three germ layers were found at 3-4 months after intramuscular administration of the mRNA-iPSCs to NOD/SCID mice.

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