PTF1a Activity in Enriched Posterior Foregut Endoderm, but Not Definitive Endoderm, Leads to Enhanced Pancreatic Differentiation in an In Vitro Mouse ESC-Based Model

Figure 6

A short 3D culture period at the beginning of differentiation promotes PTF1a-induced pancreatic differentiation. Populations that formed EB-like floating bodies either in DE or in PG stage, when replated and subjected to the differentiation protocol, expressed significantly more Amy2a (a), Insulin (b), Sst (c), and Gcg (d) transcripts than those cultures grown as monolayers throughout. Growing in suspension also improved endocrine differentiation in general (Suspension-DE No dox and Suspension-PG No dox) indicated by elevated Insulin, Sst, and Gcg transcripts. . Data are presented as mean ± SEM. Asterisks indicate values on comparison with Gelatin-No Dox cultures: , , and Obelisks indicate values on comparison with Gelatin-Dox cultures: and determined by one-way ANOVA with Tukey’s multiple comparison test.

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