Research Article

Androgen Modulates Functions of Endothelial Progenitor Cells through Activated Egr1 Signaling

Figure 4

Transwell assay of EPCs. DHT-treated or nontreated EPCs were incubated in the upper chamber with the serum-free medium. And the lower chamber was filled with 15% FBS/DMEM. Six hours later, the number of cells that migrated to the bottom of the membrane was quantified after staining with DAPI. (a) Migrating EPCs from serum-free upper chambers to the lower chambers filled with 15% FBS/DMEM. (b) The migrating EPCs were counted and averaged at high power field. All assays were triplicated and demonstrated similar results. Data are presented in mean ± SD format. versus control group.