Androgen Modulates Functions of Endothelial Progenitor Cells through Activated Egr1 Signaling

Figure 8

The transfection of EPCs by Egr1-siRNA. (a) The sequences for mouse Egr1-siRNAs. (b) After 6 h of incubation at 37°C with siRNA, transfection efficiencies can be achieved up to 90%. (c) After more 48 h of incubation at 37°C, EPCs transfected with Egr1-siRNA showed a decreased expression level of Egr-1, and the inhibition efficiencies can be up to 60%. All assays were triplicated and demonstrated similar results. Data are presented in mean ± SD format. versus control group.

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