Magnet-Bead Based MicroRNA Delivery System to Modify CD133+ Stem Cells
Uptake efficiency, viability, and surface marker pattern after transfection in supportive cytokine-supplemented culture medium. Cells were transfected with optimized miR/PEI (20 pmol miR; N/P ratio 7.5) and miR/PEI/MNP (20 pmol miR; N/P ratio 7.5; 3 and 5 μg/mL MNPs) complexes and cultured in StemSpan H3000 supplemented with StemSpan CC100. Uptake efficiency (a) was measured 18 h after transfection by flow cytometry and viability (b) and expression of CD45, CD34, and CD133+ (c) were measured 18 h and 24 h after transfection by flow cytometry. Untransfected cells were used as control. Values are presented as mean ± SEM; .