Micro- and Macrostructured PLGA/Gelatin Scaffolds Promote Early Cardiogenic Commitment of Human Mesenchymal Stem Cells In Vitro
Analysis of stemness and cardiac differentiation markers. (a) qPCR results for hMSCs cultured for 3, 4, and 15 days on monolayer microstructured PLGA/gelatin scaffolds. The expression of the same genes in 2D control cultures was used for normalization. (b) Representative confocal immunofluorescence images of c-kit and GATA-4 proteins (red) and nuclei (blue) in hMSCs after either 2D control culture (top panel) or 15-day culture (bottom panel) on microstructured bilayer PLGA/gelatin scaffolds. Magnification 63x, scale bar 20 μm.