Research Article

Micro- and Macrostructured PLGA/Gelatin Scaffolds Promote Early Cardiogenic Commitment of Human Mesenchymal Stem Cells In Vitro

Figure 8

Analysis of stemness and cardiac differentiation markers. (a) qPCR results for hMSCs cultured for 3, 4, and 15 days on monolayer microstructured PLGA/gelatin scaffolds. The expression of the same genes in 2D control cultures was used for normalization. (b) Representative confocal immunofluorescence images of c-kit and GATA-4 proteins (red) and nuclei (blue) in hMSCs after either 2D control culture (top panel) or 15-day culture (bottom panel) on microstructured bilayer PLGA/gelatin scaffolds. Magnification 63x, scale bar 20 μm.