Allogeneic Platelet Releasate Preparations Derived via a Novel Rapid Thrombin Activation Process Promote Rapid Growth and Increased BMP-2 and BMP-4 Expression in Human Adipose-Derived Stem Cells

Figure 6

Gene expression in passage 1 ASCs grown in tPR. (a) tPR treated cells displayed an increase in BMP-2 and BMP-4 expression as compared to cells grown in FBS. (b) tPR treated cells displayed a decrease in PDGF-B and FGF-2 expression as compared to cells grown in FBS. (c) Expression levels of VEGF and TGF-beta did not change. RNA was isolated from cultured cells using the BioRad Aurum minikit and rt-PCR was performed using GAPDH and RP13A as reference genes (, , and ).

(a)

(b)

(c)