Highly Efficient In Vitro Reparative Behaviour of Dental Pulp Stem Cells Cultured with Standardised Platelet Lysate Supplementation

Figure 1

Effect of PL on the growth of DPSCs. (a) Representative images from live/dead fluorescence assay after 5 days of culture in different culture conditions. Green = live; red = dead. (b) Viable counts quantified by ADAM system after 3 days of culture. Data shown as mean + SD, , statistical significance according to 2-way ANOVA method (); ns: not significant. (c) Statistic values (average viability percentages) derived from samples in (b). (d) Viability after long-term culture of 3 weeks. Data shown as mean + SD, . ns: not significant according to unpaired -test. (e) Cell growth activity measured by PrestoBlue assay. Data shown as mean + SD, . (f) CMFDA proliferation assay. Time-course comparing FBS and PL-selected condition. (g) Quantification of MFI derived from samples in (f). Statistics based on 2-way ANOVA method (). ns: not significant.

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