Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins

Figure 4

Established hNPCs express regional markers indicative of a mid/hindbrain-type property. (a) Morphologies of the expanded neurospheres at approximately passage 6 (upper panel). βIII tubulin-positive neurons after 14 days of neuronal differentiation. Green: βIII tubulin; blue: nucleus (lower panel). Scale bars: 500 μm (black) and 50 μm (white). (b) Neurite analysis after 14 days of neuronal differentiation. Relative total neurite length is shown as the mean ± SD. Statistical analysis was performed using Dunnett’s test. and . (c) Dot plots and box plots showing the expression of forebrain markers (FOXG1 and OTX1), mid/hindbrain markers (EN1 and GBX2), and a spinal cord marker (HOXC6) in day 14 (D14) neural aggregates and neurospheres at approximately passage 6 (P6). The numbers of clones analyzed are shown in parentheses. Statistical analysis was performed using Welch’s two-sample -test.. (d) Hierarchical clustering of clones based on neural progenitor marker expression. Red: high gene expression; green: low gene expression.

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