Combined MSC and GLP-1 Therapy Modulates Collagen Remodeling and Apoptosis following Myocardial Infarction

Figure 1

Effect of GLP-1 CellBeads on apoptosis and viability of ischaemic human cardiomyocytes. (a) Apoptosis was assessed using TUNEL staining. Scale bar = 50 μm. (b) 24 hours after ischaemia, MSC-GLP-1 conditioned media significantly reduced apoptosis compared to media alone. GLP-1, Exendin -4, and MSC media showed no significant reduction in apoptosis as assessed by TUNEL. (c) Cardiomyocyte viability was determined using trypan blue dye exclusion assay. GLP-1, MSC conditioned media, and MSC-GLP-1 conditioned media improved cell viability compared to media alone, 48 hours after ischaemia. (d) Ischaemic cell viability determined by cell number was increased with all agonists, compared with media alone; however this was not significant. per group versus media alone.

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