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Stem Cells International
Volume 2016, Article ID 7369491, 9 pages
Research Article

The Engrailed-1 Gene Stimulates Brown Adipogenesis

1Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
2Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
3Department of Critical Care Medicine and Emergency Room, Luhe Hospital, Capital Medical University, Beijing 101149, China

Received 25 November 2015; Revised 22 February 2016; Accepted 2 March 2016

Academic Editor: Yingmei Feng

Copyright © 2016 Chuanhai Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


As a thermogenic organ, brown adipose tissue (BAT) has received a great attention in treating obesity and related diseases. It has been reported that brown adipocyte was derived from engrailed-1 (EN1) positive central dermomyotome. However, functions of EN1 in brown adipogenesis are largely unknown. Here we demonstrated that EN1 overexpression increased while EN1 knockdown decreased lipid accumulation and the expressions of key adipogenic genes including PPARγ2 and C/EBPα and mitochondrial OXPHOS as well as BAT specific marker UCP1. Taken together, our findings clearly indicate that EN1 is a positive regulator of brown adipogenesis.