The Engrailed-1 Gene Stimulates Brown Adipogenesis

Figure 5

Knockdown of EN1 suppresses brown adipogenesis. Lentiviral mediated knockdown of EN1 expression was confirmed by (a) RT-PCR or (b) western blot analysis. Brown adipogenesis was assessed by (c) phase contrast microscopy and Oil-Red O staining. (d, e) Adipogenic and thermogenic gene expression and (f) BAT related protein expressions were analyzed during brown adipogenesis. Data are mean ± SEM. , , .

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