Effect on Multipotency and Phenotypic Transition of Unrestricted Somatic Stem Cells from Human Umbilical Cord Blood after Treatment with Epigenetic Agents

Figure 1

USSCs treated with the epigenetic agents 5-Aza-CdR and TSA stepwise change their morphology into a new spheroids-forming cell type named SpheUSSCs. The treatment of USSCs with 5-Aza-CdR and TSA first alters the USSC phenotype into a flat colony-forming cell type (pre-SpheUSSC) which after separation by trypsine and splitting and expansion of the cells alters into the stable, spheroids-forming cell type SpheUSSC.