Effect on Multipotency and Phenotypic Transition of Unrestricted Somatic Stem Cells from Human Umbilical Cord Blood after Treatment with Epigenetic Agents

Figure 2

SpheUSSCs can be spontaneously and uniformly differentiated into multivacuolar adipocytes. After cultivation with expansion medium unto the spatially confined cell expansion maximum is reached, SpheUSSCs first ceased proliferation and become sessile and then they uniformly develop multivacuolar adipocytes ((a), left and right panel), which can be stained by Oil Red O Solution ((a), right). From one middle sized cultivation flask of 75 cm² the amount of fat shown in the middle of figure’s part (a) can be isolated. Repression of transcription of the adipogenesis inhibiting factor DLK-1 accompanies the generation of SpheUSSCs and persists during adipogenic differentiation, which in addition is characterized by acquisition of LEPTIN transcription (b).

(a)

(b)