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Stem Cells International
Volume 2016, Article ID 7654321, 8 pages
http://dx.doi.org/10.1155/2016/7654321
Research Article

Epigenetic Induction of Definitive and Pancreatic Endoderm Cell Fate in Human Fibroblasts

1Interdepartmental Stem Cell Institute, Department of Development and Regeneration, Stem Cell Biology and Embryology, KU Leuven, 3000 Leuven, Belgium
2University Medical Center Groningen (UMCG), Pathology and Medical Biology, Section of Immunoendocrinology, University of Groningen, Hanzeplein 1, EA 11, 9713 GZ Groningen, Netherlands

Received 12 January 2016; Revised 22 April 2016; Accepted 8 May 2016

Academic Editor: Andrzej Lange

Copyright © 2016 Rangarajan Sambathkumar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

These supplementary figures associated with 3.Results section, 3.1 and 3.2 of the article as following order:

Figure S1: Trichostatin-A (TSA) treatment of BJ foreskin fibroblast induces transient definitive endoderm and pancreatic endoderm markers.

Figure S2: 5-azacytidine (5AZA) and Trichostatin-A (TSA) treatment of BJ foreskin fibroblast induces transient definitive endoderm and pancreatic endoderm markers.

Figure S3: Comparison of gene expression changes in primary adult fibroblasts treated with TSA-CRM vs. 5AZA-TSA-CRM.

Figure S4: Comparison of gene expression changes in BJ1 foreskin fibroblasts treated with TSA-CRM vs. 5AZA-TSA-CRM.

Figure S5: 5-azacytidine (5AZA) treatment of primary adult fibroblasts does not induce marker expression.

Figure S6: 5-azacytidine (5AZA) treatment of BJ foreskin adult fibroblast does not induces marker expression.

  1. Supplementary Material