Research Article

Gene Expression Music Algorithm-Based Characterization of the Ewing Sarcoma Stem Cell Signature

Figure 3

The stem cell signature of Ewing sarcoma cells. DNA microarray cel files from stem cells were processed as described in the caption of Figure 1. DNA microarray data from these stem cells were combined with data from a panel of neuroblastoma cell lines (NB) [1] and the Ewing sarcoma cell lines A673, EW24, and SK-N-MC [1, 3]. After RMA normalization, transformation into melodies was performed using the GEMusicA implementation in R. For this end, only the probe sets from the prefiltered stem cell signature from Figure 1 were used. The following parameters were used: , , , , , , , and . Presented is a cluster analysis of the GEMusicA-processed 1114 probe sets (Manhattan distance, complete linkage clustering).