|
| Reference | Cell source | Culture system | Supplemental factors | The yield of NCSCs | Time length | Cells induced from NCSCs in vitro | In vivo experiment | Remarks |
|
| Pomp et al., 2005 [16] | hESCs | Coculture
(PA6) | Serum | 42.3% ± 5.5% | 1 week | Peripheral sensory and sympathetic neurons | Null | First paper about NCSC induction from hESCs, but only a few molecular markers were tested |
|
| Lee et al., 2007 [17] | hESCs | Coculture
(MS-5) | Culture were switched to N2 media with serum after neural rosettes emerge from the differentiating hESCs | 30% | 1 month | Peripheral nerve system and mesenchymal stem cells | Chicken embryos and adult mice | First report using in vivo transplantation to confirm the identity of hESC-derived NCSCs, which were capable of self-renewal for several passages |
|
| Jiang et al., 2009 [18] | hESCs | Coculture
(PA6) | Serum | 55.3% ± 10.6% of the hESC colonies expressing P75 | 1 week | Peripheral neurons, glial cells, and myofibroblasts | Chicken embryos | P75+ cells after sorting showed self-renewal capacity |
|
| Hotta et al., 2009 [19] | hESCs | Coculture
(MEF) | Noggin, Y27632 | 97.7% ± 0.7% of the migrating cells were P75+ | 20 days | Enteric neurons | Quail embryos | First study reporting the use of Y27632 to induce hESCs into migrating NCSCs |
|
| Lee et al., 2010 [20] | hESCs
hiPSCs | () Coculture
(MS-5)
() 2D monolayer
(Matrigel) | Noggin, SB431542 in defined media | () 30%
() 15%40% | () 30 days
() 12 days | Peripheral neurons, Schwann cells, and myofibroblasts | Null | A feeder-free system was developed to generated NCSCs from both hESCs and hiPSCs |
|
| Menendez et al., 2011 [21] | hESCs
hiPSCs | 2D monolayer
(Geltrex) | SB431542, BIO | 80%99% | 2 weeks | Peripheral neurons and mesenchymal stem cells | Chicken embryos | This one-step protocol not only is efficient, but also generates NCSCs with stable self-renewal capacity for over 30 passages |
|
| Liu et al., 2012 [22] | hESCs
hiPSCs | 2D monolayer
(Geltrex) | PA6 conditioned media containing FGF2, Rock inhibitor, and ascorbic acid | 46% | 14 days | Peripheral neurons, Schwann cells, and mesenchymal lineages | Chicken embryos | First report showed in vitro myelination ability of Schwann cells differentiated from hESC/hiPSC-derived NCSCs |
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