Optimization of Corneal Epithelial Progenitor Cell Growth on Bombyx mori Silk Fibroin Membranes

Table 2

Relative changes in gene expression in HLE cells in response to culture in different media (). An asterisk indicates that the value is significantly different () from that obtained for the corresponding donor matched controls (DMEM).

Gene	Media	Days of culture	dCT	ddCT
p63	SHEM	7	5.95 + 0.28	−0.55 + 0.28
		14	5.3 + 0.16	−0.93 + 0.15
	DMEM	7	7.13 + 0.22	0.66 + 0.22
		14	6.97 + 0.21	0.5 + 0.21
Keratin 3	SHEM	7	6.72 + 1.15	−9.65 + 1.16
		14	3.67 + 0.82	−12.74 + 0.82
	DMEM	7	18.03 + 1.16	1.61 + 1.16
		14	14.52 + 0.29	−1.9 + 0.29
Keratin 12	SHEM	7	4.79 + 0.49	−6.46 + 0.49
		14	3.03 + 0.45	−8.2 + 0.45
	DMEM	7	12.02 + 1.46	0.77 + 1.46
		14	10.88 + 2.3	−0.36 + 2.3