Stem Cells International / 2016 / Article / Fig 3

Research Article

Fibronectin and Cyclic Strain Improve Cardiac Progenitor Cell Regenerative Potential In Vitro

Figure 3

Fibronectin increases CPC division at low strain magnitudes. CPCs were seeded on each matrix for 6 hours and then cyclic strain was applied for 24 hours. (a) Representative image showing nuclear condensation and cytokinesis. Blue: DAPI and green: FITC-maleimide. Insert shows magnification of a dividing cell. (b) Representative image of aurora B kinase staining. Blue: DAPI, green: FITC-maleimide, and red: aurora B kinase. (c) Quantified results; one-way ANOVA with Tukey’s multiple comparison test; bars represent mean + SEM; , , and ; –6; PLL (brown): poly-L-lysine, LN (green): laminin, FN (purple): fibronectin, COL (blue): collagen I, and cECM (red): naturally derived cardiac extracellular matrix.
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