Stem Cells International / 2016 / Article / Fig 5

Research Article

Fibronectin and Cyclic Strain Improve Cardiac Progenitor Cell Regenerative Potential In Vitro

Figure 5

CPC connexin43 expression is highest at low strain magnitudes. CPCs were seeded on each matrix for 6 hours and then cyclic strain was applied for 24 hours. Connexin43 expression was assessed by western blot. (a) Representative blots and (b-c) densitometry quantification. One-way ANOVA with Tukey’s multiple comparison test; bars represent mean + SEM; ; –7. (b) Matrix-dependent effects. (c) Strain magnitude-dependent effects. PLL (brown): poly-L-lysine, LN (green): laminin, FN (purple): fibronectin, COL (blue): collagen I, cECM (red): naturally derived cardiac extracellular matrix, 0–15: strain magnitude (%), and Cnx43: connexin43.

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