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Stem Cells International
Volume 2016, Article ID 8414715, 10 pages
http://dx.doi.org/10.1155/2016/8414715
Research Article

Comparison of Osteogenesis between Adipose-Derived Mesenchymal Stem Cells and Their Sheets on Poly--Caprolactone/-Tricalcium Phosphate Composite Scaffolds in Canine Bone Defects

1BK21 PLUS Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea
2College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Republic of Korea
3Powder & Ceramics Division, Korea Institute of Materials Science, Changwon 51508, Republic of Korea

Received 16 May 2016; Revised 1 July 2016; Accepted 5 July 2016

Academic Editor: Marco Tatullo

Copyright © 2016 Yongsun Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. J. Guan, J. Zhang, H. Li et al., “Human urine derived stem cells in combination with β-TCP can be applied for bone regeneration,” PLoS ONE, vol. 10, no. 5, Article ID e0125253, 2015. View at Publisher · View at Google Scholar · View at Scopus
  2. N. Kondo, A. Ogose, K. Tokunaga et al., “Bone formation and resorption of highly purified β-tricalcium phosphate in the rat femoral condyle,” Biomaterials, vol. 26, no. 28, pp. 5600–5608, 2005. View at Publisher · View at Google Scholar · View at Scopus
  3. S. A. M. Ali, S.-P. Zhong, P. J. Doherty, and D. F. Williams, “Mechanisms of polymer degradation in implantable devices. I. Poly(caprolactone),” Biomaterials, vol. 14, no. 9, pp. 648–656, 1993. View at Publisher · View at Google Scholar · View at Scopus
  4. G. Wei and P. X. Ma, “Structure and properties of nano-hydroxyapatite/polymer composite scaffolds for bone tissue engineering,” Biomaterials, vol. 25, no. 19, pp. 4749–4757, 2004. View at Publisher · View at Google Scholar · View at Scopus
  5. K. Fujihara, M. Kotaki, and S. Ramakrishna, “Guided bone regeneration membrane made of polycaprolactone/calcium carbonate composite nano-fibers,” Biomaterials, vol. 26, no. 19, pp. 4139–4147, 2005. View at Publisher · View at Google Scholar · View at Scopus
  6. R. L. Simpson, F. E. Wiria, A. A. Amis et al., “Development of a 95/5 poly(L-lactide-co-glycolide)/hydroxylapatite and β-tricalcium phosphate scaffold as bone replacement material via selective laser sintering,” Journal of Biomedical Materials Research B: Applied Biomaterials, vol. 84, no. 1, pp. 17–25, 2008. View at Publisher · View at Google Scholar · View at Scopus
  7. B.-J. Kang, H.-H. Ryu, S. S. Park et al., “Comparing the osteogenic potential of canine mesenchymal stem cells derived from adipose tissues, bone marrow, umbilical cord blood, and Wharton's jelly for treating bone defects,” Journal of Veterinary Science, vol. 13, no. 3, pp. 299–310, 2012. View at Publisher · View at Google Scholar · View at Scopus
  8. M. Yamato and T. Okano, “Cell sheet engineering,” Materials Today, vol. 7, no. 5, pp. 42–47, 2004. View at Publisher · View at Google Scholar · View at Scopus
  9. M. Akahane, A. Nakamura, H. Ohgushi, H. Shigematsu, Y. Dohi, and Y. Takakura, “Osteogenic matrix sheet-cell transplantation using osteoblastic cell sheet resulted in bone formation without scaffold at an ectopic site-,” Journal of Tissue Engineering and Regenerative Medicine, vol. 2, no. 4, pp. 196–201, 2008. View at Publisher · View at Google Scholar · View at Scopus
  10. T. Long, Z. Zhu, H. A. Awad, E. M. Schwarz, M. J. Hilton, and Y. Dong, “The effect of mesenchymal stem cell sheets on structural allograft healing of critical sized femoral defects in mice,” Biomaterials, vol. 35, no. 9, pp. 2752–2759, 2014. View at Publisher · View at Google Scholar · View at Scopus
  11. H.-H. Ryu, J.-H. Lim, Y.-E. Byeon et al., “Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury,” Journal of Veterinary Science, vol. 10, no. 4, pp. 273–284, 2009. View at Publisher · View at Google Scholar · View at Scopus
  12. M. Neupane, C.-C. Chang, M. Kiupel, and V. Yuzbasiyan-Gurkan, “Isolation and characterization of canine adipose-derived mesenchymal stem cells,” Tissue Engineering A, vol. 14, no. 6, pp. 1007–1015, 2008. View at Publisher · View at Google Scholar · View at Scopus
  13. N. M. Vieira, V. Brandalise, E. Zucconi, M. Secco, B. E. Strauss, and M. Zatz, “Isolation, characterization, and differentiation potential of canine adipose-derived stem cells,” Cell Transplantation, vol. 19, no. 3, pp. 279–289, 2010. View at Publisher · View at Google Scholar · View at Scopus
  14. C. A. Gregory, W. G. Gunn, A. Peister, and D. J. Prockop, “An Alizarin red-based assay of mineralization by adherent cells in culture: comparison with cetylpyridinium chloride extraction,” Analytical Biochemistry, vol. 329, no. 1, pp. 77–84, 2004. View at Publisher · View at Google Scholar · View at Scopus
  15. K. J. Livak and T. D. Schmittgen, “Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method,” Methods, vol. 25, no. 4, pp. 402–408, 2001. View at Publisher · View at Google Scholar · View at Scopus
  16. T. L. Arinzeh, S. J. Peter, M. P. Archambault et al., “Allogeneic mesenchymal stem cells regenerate bone in a critical-sized canine segmental defect,” The Journal of Bone & Joint Surgery—American Volume, vol. 85, no. 10, pp. 1927–1935, 2003. View at Google Scholar · View at Scopus
  17. S. P. Bruder, K. H. Kraus, V. M. Goldberg, and S. Kadiyala, “The effect of implants loaded with autologous mesenchymal stem cells on the healing of canine segmental bone defects,” The Journal of Bone & Joint Surgery—American Volume, vol. 80, no. 7, pp. 985–996, 1998. View at Google Scholar · View at Scopus
  18. Q. Xie, Z. Wang, Y. Huang et al., “Characterization of human ethmoid sinus mucosa derived mesenchymal stem cells (hESMSCs) and the application of hESMSCs cell sheets in bone regeneration,” Biomaterials, vol. 66, pp. 67–82, 2015. View at Publisher · View at Google Scholar · View at Scopus
  19. J. Yu, Y.-K. Tu, Y.-B. Tang, and N.-C. Cheng, “Stemness and transdifferentiation of adipose-derived stem cells using l-ascorbic acid 2-phosphate-induced cell sheet formation,” Biomaterials, vol. 35, no. 11, pp. 3516–3526, 2014. View at Publisher · View at Google Scholar · View at Scopus
  20. M. Akahane, H. Shigematsu, M. Tadokoro et al., “Scaffold-free cell sheet injection results in bone formation,” Journal of Tissue Engineering and Regenerative Medicine, vol. 4, no. 5, pp. 404–411, 2010. View at Publisher · View at Google Scholar · View at Scopus
  21. A. Nakamura, M. Akahane, H. Shigematsu et al., “Cell sheet transplantation of cultured mesenchymal stem cells enhances bone formation in a rat nonunion model,” Bone, vol. 46, no. 2, pp. 418–424, 2010. View at Publisher · View at Google Scholar · View at Scopus
  22. R. C. Lai, S. S. Tan, B. J. Teh et al., “Proteolytic potential of the MSC exosome proteome: implications for an exosome-mediated delivery of therapeutic proteasome,” International Journal of Proteomics, vol. 2012, Article ID 971907, 14 pages, 2012. View at Publisher · View at Google Scholar
  23. Y.-E. Byeon, H.-H. Ryu, S. S. Park et al., “Paracrine effect of canine allogenic umbilical cord blood-derived mesenchymal stromal cells mixed with beta-tricalcium phosphate on bone regeneration in ectopic implantations,” Cytotherapy, vol. 12, no. 5, pp. 626–636, 2010. View at Publisher · View at Google Scholar · View at Scopus
  24. M. Pensak, S. Hong, A. Dukas et al., “The role of transduced bone marrow cells overexpressing BMP-2 in healing critical-sized defects in a mouse femur,” Gene Therapy, vol. 22, no. 6, pp. 467–475, 2015. View at Publisher · View at Google Scholar · View at Scopus
  25. G. Chen, C. Deng, and Y.-P. Li, “TGF-β and BMP signaling in osteoblast differentiation and bone formation,” International Journal of Biological Sciences, vol. 8, no. 2, pp. 272–288, 2012. View at Publisher · View at Google Scholar · View at Scopus
  26. S. Vanhatupa, M. Ojansivu, R. Autio, M. Juntunen, and S. Miettinen, “Bone morphogenetic protein-2 induces donor-dependent osteogenic and adipogenic differentiation in human adipose stem cells,” Stem Cells Translational Medicine, vol. 4, no. 12, pp. 1391–1402, 2015. View at Publisher · View at Google Scholar · View at Scopus
  27. T. Reya and H. Clevers, “Wnt signalling in stem cells and cancer,” Nature, vol. 434, no. 7035, pp. 843–850, 2005. View at Publisher · View at Google Scholar · View at Scopus
  28. H.-S. Yun, S.-H. Kim, D. Khang, J. Choi, H.-H. Kim, and M. Kang, “Biomimetic component coating on 3D scaffolds using high bioactivity of mesoporous bioactive ceramics,” International Journal of Nanomedicine, vol. 6, pp. 2521–2531, 2011. View at Google Scholar · View at Scopus
  29. G. Marino, F. Rosso, G. Cafiero et al., “β-Tricalcium phosphate 3D scaffold promote alone osteogenic differentiation of human adipose stem cells: in vitro study,” Journal of Materials Science: Materials in Medicine, vol. 21, no. 1, pp. 353–363, 2010. View at Publisher · View at Google Scholar · View at Scopus
  30. P. Müller, U. Bulnheim, A. Diener et al., “Calcium phosphate surfaces promote osteogenic differentiation of mesenchymal stem cells,” Journal of Cellular and Molecular Medicine, vol. 12, no. 1, pp. 281–291, 2008. View at Publisher · View at Google Scholar · View at Scopus