Human Liver Stem Cells Suppress T-Cell Proliferation, NK Activity, and Dendritic Cell Differentiation

Figure 5

HLSCs suppress monocyte-derived DC differentiation and DC ability to stimulate T-cell proliferation. (a) Mean percentages ± SD of the expression of CD14, CD83, CD40, CD80, CD86, HLA-DR, CD1a, CD54, and α4 and α5 integrin of DCs differentiated in the presence of HLSCs or MSCs or in the absence of stem cells (CTRL) evaluated by cytofluorimetric analyses. Results were obtained from 8 independent experiments. ANOVA with Dunnet’s multicomparison test was performed: stem cells versus CTRL; stem cells versus CTRL. (b) Mean fluorescence intensity expressed as GEO mean ± SD of CD40, CD1a, CD80, α5 integrin, CD86, HLA-DR, and CD54 of DCs differentiated in the presence of HLSCs or MSCs or in the absence of stem cells (CTRL). Results were obtained from 8 independent experiments. ANOVA with Dunnet’s multicomparison test was performed: stem cells versus CTRL; stem cells versus CTRL. (c) DCs differentiated in the presence of HLSCs or in the absence were plated at cell concentration of 2 × 10⁴ with 1 × 10⁵ T CD3⁺ lymphocytes. Forty-eight hours later, T-cell proliferation was assessed. Data are expressed as mean ± SD of percent variation of T-cell proliferation in the presence or the absence of DCs differentiated in the presence of HLSCs with respect to T-cell proliferation in the presence of DCs alone (established as 100%). Results were obtained from 5 independent experiments. Data were analysed by Student’s -test (unpaired, 2-tailed); DC (HLSC)+T versus DC+T.

(a)

(b)

(c)