Characterization and Expression of Senescence Marker in Prolonged Passages of Rat Bone Marrow-Derived Mesenchymal Stem Cells

Figure 2

Characterization of rBM-MSCs. The immunophenotyping to characterize the surface markers was performed using rBM-MSC from passage 3. Cells were positively expressed as CD90.1, CD29, CD71, and CD44 and negatively expressed as CD11b/c and CD45 (a). Mesodermal differentiation was conducted using cells of passage 3 that is subjected to the relevant induction media. After 20 days of induction, cells were stained with Oil Red O solution and Alizarin Red solution, respectively. Adipogenic differentiation was evidence by lipid droplet formation stained with Oil Red O whereas osteogenic differentiation was evidenced by calcium deposits stained with Alizarin Red (b). Gene expression of differentiated adipocytes and osteocytes was evaluated using RT-PCR. Untreated rBM-MSC (control) showed faint expression for osteocytes (c). Results were representative of 3 experiments. OP: osteopontin, ON: osteonectin, PP: PPARγ, and CE: C/EBPA. Microscopic magnification: 200x.

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