Characterization and Expression of Senescence Marker in Prolonged Passages of Rat Bone Marrow-Derived Mesenchymal Stem Cells

Table 3

PCR gene amplification conditions for GAPDH, osteopontin, osteonectin, PPARγ, and C/EBPA gene for adipogenesis and osteogenesis differentiation assays.

Process	Temperature (°C)	Time	Cycle
Predenaturation	95	3 min	1
Denaturation	95	30 sec	35
Annealing		1 min	35
GAPDH	48.1
Osteopontin	57.8
Osteonectin	60.5
PPARγ	47.3
C/EBPA	66.7
Elongation	72	1 min	35
Final elongation	72	10 min	1
Incubation	10	∞	∞