Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells

Figure 6

(a): Scatter plot of ratios for the two differential analyses of reprogramming versus hFibs (-axis) and haGSCs versus hESCs (-axis). Grey dashed lines represent the thresholds of −2 and 2 used definition of the sectors. Different sectors are labelled with blue-coloured Roman numerals. The plot is divided into nine different sectors taking ratios of −2 and 2 as thresholds which corresponds to a 4-fold differential regulation. The nine sectors were as follows. Sector I 798 genes were upregulated in 157-23_P5 compared to hFibs and upregulated in 157-23_P5 compared to hESCs. Sector II 895 genes were not differentially regulated in 157-23_P5 compared to hFibs and upregulated in 157-23_P5 compared to hESCs. Sector III 8 genes were downregulated in 157-23_P5 compared to hFibs and upregulated compared to hESCs. Sector IV 454 genes were upregulated in 157-23_P5 compared to hFibs and not differentially regulated in 157-23_P5 compared to hESCs. Sector V 49468 genes are not differentially regulated in 157-23_P5 compared to hFibs and not differentially regulated in 157-23_P5 compared to hESCs. Sector VI 855 genes are downregulated in 157-23_P5 compared to hFibs and not differentially regulated in 157-23_P5 compared to hESCs. Sector VII 2 genes are upregulated in 157-23_P5 compared to hFibs and downregulated in 157-23_P5 compared to hESCs. Sector VIII 1744 genes are not differentially regulated in 157-23_P5 compared to hFibs and downregulated in 157-23_P5 compared to hESCs. Sector IX 451 genes are downregulated in 157-23_P5 compared to hFibs and downregulated in 157-23_P5 compared to hESCs. The majority of the genes were not differentially expressed between 157-23_P5, hFibs, and hESCs (49K gene in Sector V). (b) Heatmap with pluripotency associated genes upregulated by haGSCs in comparison to hFibs according to microarray experiment. (c) Gene expression profiling of hESCs, haGSCs, and hFibs of additional pluripotency associated genes upregulated according to microarray study in haGSCs. (a) haGSCs are a heterogenic population of cells with some similarities to hESCs (coloured black) and hSSCs (coloured aquamarine) but not to hFibs (coloured red).

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