Highly Synchronized Expression of Lineage-Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines

Figure 6

Gene expression profiles of early hepatic markers (TBX3, HNF4A, HNF6, and AFP) in the interval from day 7 to day 35. (a) The -axis indicates the days during differentiation and the -axis indicates RQ, where the calibrator’s RQ = 1. The colored lines show the results from each individual hPSC line and the grey line indicates the calculated median value of the RNA level. Correlation tables are shown in (b). The graphs show synchronicity of onset as well as up- and downregulation among all cell lines for the genes TBX3, HNF4A, HNF6, and AFP. The average correlation between pairs of cell lines ranges from moderate (0.52 for HNF4A) to very strong (0.83 for TBX3).