Figure 1: Differentiation of hESCs to hESC-Fib. (a–d) The photomicrographs demonstrate the differentiation of hESCs to embryoid bodies, embryoid body outgrowth, and hESC-Fib under high-glucose differentiation conditions. (e) Characterization of hESC-Fib by real-time RT-PCR for pluripotency (OCT4, SOX2, and NANOG) and fibroblast-related transcripts (COL1A1, COL3A1, P4Hβ, and VIMENTIN). (f) Photomicrograph shows the spindle-shaped hPLFs. Scale bar: 500 µm.