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Stem Cells International
Volume 2016, Article ID 9013089, 10 pages
Research Article

Differentiation and Molecular Properties of Mesenchymal Stem Cells Derived from Murine Induced Pluripotent Stem Cells Derived on Gelatin or Collagen

Research Center for Radiation Emergency Medicine, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba-shi, Chiba 263-8555, Japan

Received 2 February 2016; Revised 9 July 2016; Accepted 19 July 2016

Academic Editor: Pavla Jendelova

Copyright © 2016 Chizuka Obara et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Table 1 showed primers for PDGFRα, PDGFRβ, actin, and SOX1. Supplementary Table 2 showed all antibodies in this study. Supplementary Figure 1 showed removal of MEF cells using feeder removal microbeads and LC column. Supplementary Figure 2 showed microarray analysis of 78 gene expression profile. Supplementary Figure 3 showed clustering analysis by selecting 50 genes that were upregulated by two-fold in BMMSCs relative to iPSCs. Supplementary Figure 4 showed osteogenic and adipogenic differentiation of iMSC/G an C derived from 2A-4F-100 and 2A-4F-136 iPSC cell lines.

  1. Supplementary Material