NK Cell and CD4+FoxP3+ Regulatory T Cell Based Therapies for Hematopoietic Stem Cell Engraftment

Table 1

Treg adoptive transfer in clinical trials.

	HCT type	Clinical approach	Patients treated	Treg isolation	Treg number/kg	GvHD outcome
Trzonkowski et al., 2009 [78]	HLA-matched	T: aGvHD and cGvHD	2 (1 aGvHD and 1 cGvHD)	Magnetic separation; FACS sorting; In vitro expansion	cGvHD (1 × 10^5); aGvHD (2 × 10^6)	1 (cGvHD) response
Brunstein et al., 2011 [75]	Cord blood	P	23	Magnetic separation; In vitro expansion	1 × 10^5–3 × 10^6	aGvHD II–IV (43%); aGvHD III-IV (17%); cGvHD (14%)
Di Ianni et al., 2011 [76]	Haploidentical	P	28	Magnetic separation	2 × 10^6–4 × 10^6	aGvHD (2/26); No reported cGvHD
Martelli et al., 2014 [77]	Haploidentical	P	43	Magnetic separation	2 × 10^6	aGvHD (6/41); cGvHD (1/41)
Theil et al., 2015 [79]	HLA-matched (4); Cw mismatch (1)	T: cGvHD	5	Magnetic separation; In vitro expansion	Median 2.36 × 10^6	Response (2); Stable (3)

T: treatment; P: prevention.