Three-Dimensional Aggregates Enhance the Therapeutic Effects of Adipose Mesenchymal Stem Cells for Ischemia-Reperfusion Induced Kidney Injury in Rats

Figure 3

3D aggregates decrease the susceptibility of AdMSCs to stress and enhance the expression of proangiogenic factors in AdMSCs. (a) Representative FDA/PI stained images of 2D AdMSCs or 3D aggregates subjected to hypoxia/reoxygenation or 200 μM-H₂O₂ treatment. (b) Quantification of dead cells in 2D AdMSCs or 3D aggregates subjected to hypoxia/reoxygenation treatment using PI/FDA ratio. Data were normalized to the vehicle group of 2D monolayer culture. (c) Quantification of dead cells in 2D AdMSCs or 3D aggregates subjected to 200 μM-H₂O₂ treatment using PI/FDA ratio. Data were normalized to the vehicle group of 2D monolayer culture. (d) Relative mRNA expression of VEGF, FGF2, and HGF in 2D AdMSCs and 3D aggregates. compared with 2D AdMSCs.

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