Expression of the Wnt Receptor Frizzled-4 in the Human Enteric Nervous System of Infants

Figure 2

Frizzled-4 is detected in a subpopulation of neural cells. Immunofluorescence for frizzled-4 (Fzd4), , nestin, peripherin, GFAP, and c-kit on cryostat sections of human ileum (sample number 2). ((a1)–(a3)) Combined (green) and Fzd4 (red) immunostaining of myenteric ganglia; (a1); Fzd4 (a2); merge (a3); DAPI (blue). ((b1)–(b3)) Combined nestin (green) and Fzd4 (red) immunostaining of myenteric ganglia; nestin (b1); Fzd4 (b2); merge (b3); DAPI (blue). ((c1)–(c3)) Combined peripherin (green) and Fzd4 (red) immunostaining of myenteric ganglia; peripherin (c1); Fzd4 (c2); merge (c3); DAPI (blue). (d1–d3) Combined GFAP (green) and Fzd4 (red) immunostaining of myenteric ganglia; GFAP (d1); Fzd4 (d2); merge (d3); DAPI (blue). ((e1)–(e3)) Combined c-kit (green) and Fzd4 (red) immunostaining of myenteric ganglia; c-kit (e1); Fzd4 (e2); merge (e3); DAPI (blue); scale bar (a–e): 100 μm.