Expression of the Wnt Receptor Frizzled-4 in the Human Enteric Nervous System of Infants

Figure 3

Epithelial crypts of the mucosa are surrounded by frizzled-4 positive neural extensions. Immunofluorescence of frizzled-4 (Fzd4) on cryostat sections of human ileum; (sample number 2). ((a1)–(a3)) Sagittal section. Combined Fzd4 (red) and DAPI (blue) fluorescence staining (a1); combined Fzd4 fluorescence (red) and brightfield view (a2); merge (a3). The broken lines mark the border between epithelial cells of the crypt base and the lamina propria mucosae. The white arrowheads indicate the borders of the lamina muscularis mucosae (LMM) to the lamina propria mucosae and the tela submucosa (TSM); scale bar: 50 μm. ((b1)–(b3)) Horizontal section of the crypt region. Combined Fzd4 (red) and DAPI (blue) fluorescence staining (b1); combined Fzd4 (red) fluorescence and brightfield view (b2); merge (b3). The broken lines mark the border between epithelial cells of some crypts and the lamina propria mucosae; scale bar (a, b): 50 μm.