Expression of the Wnt Receptor Frizzled-4 in the Human Enteric Nervous System of Infants

Figure 4

Identification of frizzled-4 (Fzd4) mRNA by RT-PCR. Total RNA was isolated from tunica muscularis. Respective primer pairs were used for detection of Fzd4 (605 bp) and the housekeeping gene GAPDH (452 bp). Isolated RNA without subsequent reverse transcriptase step served as negative control.