Expression of the Wnt Receptor Frizzled-4 in the Human Enteric Nervous System of Infants

Table 2

Primary antibodies used for cell analysis.

Primary antibodies	Species	Dilution	Manufacturer
α-smooth muscle actin	Rabbit	1 : 100	Spring Bioscience, Pleasanton, USA
c-kit	Rabbit	1 : 500	Dako, Hamburg, Germany
Fzd4 (clone CH3A4)	Mouse	Undiluted	In-house (HJB)
Fzd4 PE (clone CH3A4)	Mouse	1 : 50	Biolegend, San Diego, USA
GFAP	Rabbit	1 : 400	Dako, Hamburg, Germany
Nestin	Rabbit	1 : 1000	Abcam, Cambridge, UK
p75^NTR	Rabbit	1 : 500	Promega, Madison, USA
p75^NTR APC	Rabbit	1 : 50	Miltenyi, Bergisch Gladbach, Germany
Peripherin	Rabbit	1 : 200	Merck Millipore, Darmstadt, Germany

Monoclonal mouse anti-human antibody CH3A4 against frizzled-4 was raised by immunization with the retinoblastoma cell line WERI-RB-1 and specificity for frizzled-4 was verified by the selective recognition of HEK-293 cells transfected with human frizzled-4. This molecule was clustered to CD344 at the HCDM workshop in Quebec, Canada (http://www.hcdm.org/).