Stem Cell Imaging: Tools to Improve Cell Delivery and Viability

Figure 1

Procedure for SCT. Cells can be labeled with contrast agent either directly or indirectly. The labeled cells are purified from unlabeled cells to obtain a cell product with high signal and thus contrast versus adjacent tissue. Before the delivery, the stability of the labeled cells can be tested to assess any potential toxicity of the imaging agent. After delivery, the viability of the delivered cells is monitored to understand engraftment and survival. The labeled stem cells can be clearly recognized due to increased signal produced by the label. Finally, histology and associated microscopy techniques can confirm that the imaging signal does indeed correspond to the cells of interest.