Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton’s Jelly-Derived Mesenchymal Stem Cells
Morphology and expression of neural-specific genes and protein in WJ MSCs (Wharton’s jelly-derived mesenchymal stem cells) in the early and middle phases after neural induction. (a and d) After DMSO and BHA (β-hydroxyanisole) treatment, WJ MSCs in the early and middle phases exhibited a neuronal-like morphology. Bar: 100 μm. (b, c, e, f) Immunocytofluorescence staining results showed that neural induction significantly increased the protein expression of Nestin and β-Tubulin III in induced cells (induced P7 and induced P14). Bar: 50 μm. (g) The gene expression of Nestin and β-Tubulin III was upregulated after neural induction with no significant differences between two groups (induced P7 and induced P14). (h) The percentage of β-Tubulin III positive cells in the group of P14 induction () was significantly higher than P7 induction () according to the statistical analysis results (), which indicated that serial passage significantly increased the neural differentiation capability of WJ MSCs.
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