Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton’s Jelly-Derived Mesenchymal Stem Cells

Table 1

Real-time PCR primers used in the experiments.

Target	Forward primer	Reverse primer	Genbank acc. number
p21	5′-GGACAGCAGAGGAAGACCATGT-3′	5′-CGGCGTTTGGAGTGGTAGAA-3′	NM_000389
E-Ras	5′-GCAAGAGTGCGCTGACCAT-3′	5′-GCCCAGCACACCATCACA-3′	NM_181532.3
c-Myc	5′-CCACAGCAAACCTCCTCACA-3′	5′-CGGTTGTTGCTGATCTGTCTCA-3′	NM_002467
Oct3/4	5′-GTGGAGGAAGCTGACAACAATGAAA-3′	5′-GACCGAGGAGTACAGTGCAGTGAAG-3′	NM_002701
Nanog	5′-CAAAGGCAAACAACCCACTT-3′	5′-ATTGTTCCAGGTCTGGTTGC-3′	XM_002344636.1
Vimentin	5′-GGTGGACCAGCTAACCAACGA-3′	5′-TCAAGGTCAAGACGTGCCAGA-3′	NM_003380.3
Gata4	5′-GTTTTTTCCCCTTTGATTTTTGATC-3′	5′-AACGACGGCAACAACGATAAT-3′	NM_002052
Nestin	5′-GGGTCTACAGAGTCAGATCGCTCA-3′	5′-AGCGAGAGTTCTCAGCCTCCA-3′	NM_006617
AFP	5′-TAAACCCTGGTGTTGGCCAG-3′	5′-ATTTAAACTCCCAAAGCAGCAC-3′	NM_001134
Nkx2.5	5′-CCCCTGGATTTTGCATTCAC-3′	5′-CGTGCGCAAGAACAAACG-3′	NM_004387
β-Tubulin III	5′-CCTATTCAGGCCCGACAACTTTA-3′	5′-CAGGCAGTCACAATTCTCACACTC-3′	NM_006086
β-actin	5′-TGGCACCCAGCACAATGAA-3′	5′-CTAAGTCATAGTCCGCCTAGAAGCA-3′	NM_001101