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Figure 2: Differentiation of rat bone marrow mesenchymal stem cells (BMSCs) into adipocytes and osteoblasts. Isolated rat BMSCs were grown in either adipogenic or osteogenic induction media, and their adipogenic and osteogenic differentiation abilities were assessed by oil red O staining (a) and alizarin red staining (b), respectively. (c) Phenotypic characterization of the rat BMSCs at passage 3. (d) Effect of PRP on the proliferation of rat BMSCs. Rat BMSCs were cultured in complete growth media with or without PRP for 48 h, and the proliferation of cells was measured by MTS assays. (e) The colony-forming efficiency of rat BMSCs is enhanced by PRP treatment. The cells were cultured in complete growth media with or without PRP for 2 weeks, and the colony-forming efficiency was determined by the colony formation assay. (f) Effects of PRP on the adipogenic (lower panel) and osteogenic (upper panel) differentiation potentials in rat BMSCs.