Research Article

Comparison of Immunomodulation Properties of Porcine Mesenchymal Stromal/Stem Cells Derived from the Bone Marrow, Adipose Tissue, and Dermal Skin Tissue

Figure 3

In vitro proliferation capability of passage 3 mesenchymal stromal/stem cells (MSCs). (A) and (B) display growth rate (absolute growth rate and doubling time in a & b, resp.) parameters of MSCs and the cell cycle, respectively. For analysis of the growth rate, MSCs were seeded at cells/well in 24-well tissue culture plates, and the number of cells was calculated at 48 h intervals. For cell cycle analysis, MSCs were seeded at a density of cells per 35 mm dish, cultured until ca. 80% confluence, and stained with propidium iodide (PI). The first red line, the second blue oblique lines, and the third red-filled peak indicate the G0/G1, S, and G2/M phases, respectively. Experiments shown in (A) and (B) were performed in triplicate.