Research Article

Comparison of Immunomodulation Properties of Porcine Mesenchymal Stromal/Stem Cells Derived from the Bone Marrow, Adipose Tissue, and Dermal Skin Tissue

Figure 4

Analysis of OCT3/4 and TERT in passage 3 mesenchymal stromal/stem cells (MSCs). (A) RT-PCR for OCT4 and TERT. (B) Western blot of OCT3/4 and TERT. Porcine testis and MRC5 cells were used as positive control and negative control for both OCT4 and TERT, respectively. F9 and HeLa cells were used as positive controls for OCT3/4 and TERT, respectively. The internal control was GAPDH for RT-PCR and β-actin for western blot. (C) Protein expression of OCT3/4, measured by flow cytometry, was performed in triplicate ((C)a). . ((C)b) black and green open histograms indicate negative and positive immunoreactivity, respectively.