Comparison of Immunomodulation Properties of Porcine Mesenchymal Stromal/Stem Cells Derived from the Bone Marrow, Adipose Tissue, and Dermal Skin Tissue

Figure 6

Tumor formation in mesenchymal stromal/stem cells (MSCs) transplanted into immunodeficient mice. (A) and (B) Images obtained after elimination of dorsal hair or clothing, respectively, in each mouse. (a–f) Cells () stained with PKH26 were transplanted to both dorsal subcutaneous spaces of ICR mice (a) or NOD/SCID mice (b–f), and the mice were sacrificed after 15 weeks to confirm tumor formation. ((A)a-b) and ((B)a-b) show mice injected with MDA-MB231 as a positive control and c–f show mice injected with PBS + Matrigel (M), A-MSCs + M, BM-MSCs + M, and DS-MSCs + M, respectively. (C) and (D) display tumors isolated from mouse (A)b (Figures 6(A)-6(b)). ((E)a–c) Immunohistochemical staining and ((E)d) H&E staining were performed in tissue sections from tumors. ((E)a) and ((E)b) Red and blue identify the PKH26-stained membrane of cells and counterstaining of nucleic acids, respectively. Arrows indicate the blood vessels. Scale bars = 500 μm.