Research Article

Periodontal Ligament Stem Cells in the Periodontitis Microenvironment Are Sensitive to Static Mechanical Strain

Figure 3

Effects of different magnitudes of SMS on the osteogenesis of HPDLSCs and PPDLSCs. (a) ALP activity was detected by ALP staining. (b) Quantitative analysis of ALP activity. (c) Osteogenic differentiation was determined by Alizarin Red S staining. (d) Quantitative analysis of calcium concentration was performed by calcium level analysis. (e) The expression levels of the osteogenic genes Runx2, ALP, and OPG were measured by real-time PCR. versus the other five groups of HPDLSCs or PPDLSCs; in each group. Each experiment was performed three times. The data are presented as the mean ± standard deviation.
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