From Blood to Lesioned Brain: An In Vitro Study on Migration Mechanisms of Human Nasal Olfactory Stem Cells

Figure 6

Mouse recombinant mCcl2 and mSpp1 cytokines also stimulate human OE-MSC migration. Effects of mouse recombinant cytokines (i.e., mCcl2 and mSpp1) on human OE-MSCs were assessed using modified Boyden chambers (a) and agarose spot assays (b). Mean percentage of migrating human OE-MSCs per field in response to buffer or cytokines at increasing concentrations, 12 hours after the start of the experiment (a). Mean number of migrating human OE-MSCs per drop in response to buffer or cytokines at 10 μg/mL, 24 hours after the start of the experiment (b). Values reported are the mean (±SEM) of at least three independent experiments performed in triplicate for modified Boyden chambers and in ten replicate drops for agarose spot assays. (, , and , using Student’s t-test.)

(a)

(b)