From Blood to Lesioned Brain: An In Vitro Study on Migration Mechanisms of Human Nasal Olfactory Stem Cells

Figure 7

CCL2 stimulates both chemokinesis and chemotaxis while SPP1 mainly acts on chemokinesis. Random cell motility (chemokinesis) and gradient-dependent cell migration (chemotaxis) of human OE-MSCs in response to human recombinant cytokines hCCL2 and hSPP1 were assessed using modified Boyden chambers (a, c) and agarose spot assays (b, d). (a, c) Mean percentage of migrating human OE-MSCs per field, 12 hours later, in response to buffer or cytokines placed either in the lower well (below) or in both the upper and the lower wells (above and below). (b, d) Mean number of migrating human OE-MSCs per drop, 24 hours later, in response to buffer or cytokines placed either in the agarose drop or in both the medium and the drop. Values reported are the mean (±SEM) of at least three independent experiments performed in triplicate for modified Boyden chambers and in ten replicate drops for agarose spot assays. (, , and , using Student’s t-test.)

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