From Blood to Lesioned Brain: An In Vitro Study on Migration Mechanisms of Human Nasal Olfactory Stem Cells

Figure 8

Expression of CCR receptors in human OE-MSCs suggests CCL2 acts through unsuspected pathways. (a–d) mRNA expression of C-C chemokine receptors (CCRs) that can potentially bind CCL2 (i.e., CCR2, CCR1, CCR4, and CCR10), normalized to the ABELSON housekeeping gene (ABL1). Values reported are the mean (±SEM) of at least three independent experiments performed in duplicate. Expression level of each gene is represented by 2^−∆CT where ∆CT = CT (gene X) − CT (ABL1). Human OE-MSCs from three donors (donors 1, 2, and 3) were analyzed. The acute monocytic leukemia cell line THP1 was used as positive control. ND: not detected.

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